The synthesis of coenzyme A from panthetheine: preparation and properties of panthetheine kinase.

It had previously been shown (l-3) that extracts of pigeon liver could convert pantetheine and ATP to CoA’ in low yield. It was found, however, that fresh extracts of pigeon liver, from which CoA has been removed with Dowex 1 rather than by aging, effected a much more vigorous synthesis of CoA. Since the aging procedure apparently inactivated the enzyme responsible for the conversion of pantetheine to phosphopantetheine, purification of this enzyme was carried out starting with freshly prepared extracts.